Question
Explain the steps involved in subcellular fractionation.
Answer :
Word Count : 236
____ ________ __________ _________ ______ _____ _____ _______ ___ __________ ___ _________.
_________ _________ ______ _______ ________ _____ _____ ________ ________.
______ ______ _____ __________ ___ ______ __________ ___ ________ ______.
__________ _________ _____ _________ ________ ______ ______.
______ ___ _________ ___ ____ _______ _____.
______ _________ _______ ________ ____ __________ ________ ______.
_____ _____ _________ _______ _______ ________ _______.
___ ______ __________ ___ ______ ________ _____ _______ _________ _________ ________ _________.
_______ ______ ______ ___ ______ ____.
________ _______ __________ _____ ______ __________.
_______ ___ ___ ______ ______ ___ ______ _____.
_________ __________ _______ ________ _________ ________ ___ ________ ________ ___ ______ ___.
___ _____ _______ _____ ______ ______.
_______ ____ ____ __________ ________ _____ ______ __________.
____ _______ ___ ___ _____ ___.
______ ____ ____ ________ ___ _________ ___ _____ _________ ___ __________ ____.
_____ _______.
Skip in 10
Subcellular fractionation is a technique used in cell biology to separate cellular components based on their size, density, and sedimentation properties. The process begins with the careful collection of cells from tissues or cultured cell lines. The cells are first washed with an isotonic buffer to remove extracellular contaminants and then subjected to homogenization. Homogenization involves physically breaking open the cells using methods such as grinding, blending, or sonication, while maintaining conditions that preserve the integrity of organelles. This produces a uniform suspension called the cell homogenate.
____ __________ _________ _________ _________ ______ _________ ________ _________ _________ _____.____ ________ __________ _________ ______ _____ _____ _______ ___ __________ ___ _________.
_________ _________ ______ _______ ________ _____ _____ ________ ________.
______ ______ _____ __________ ___ ______ __________ ___ ________ ______.
__________ _________ _____ _________ ________ ______ ______.
______ ___ _________ ___ ____ _______ _____.
______ _________ _______ ________ ____ __________ ________ ______.
_____ _____ _________ _______ _______ ________ _______.
___ ______ __________ ___ ______ ________ _____ _______ _________ _________ ________ _________.
_______ ______ ______ ___ ______ ____.
________ _______ __________ _____ ______ __________.
_______ ___ ___ ______ ______ ___ ______ _____.
_________ __________ _______ ________ _________ ________ ___ ________ ________ ___ ______ ___.
___ _____ _______ _____ ______ ______.
_______ ____ ____ __________ ________ _____ ______ __________.
____ _______ ___ ___ _____ ___.
______ ____ ____ ________ ___ _________ ___ _____ _________ ___ __________ ____.
_____ _______.
IGNOU ACADEMY
Your IGNOU journey, made easier.
- Solved Assignments
- Study Notes
- Guess Papers
- Question Papers
Continue Reading — It's Free
Sign in with Google to unlock more of this answer.
✓ Free access
✓ Unlock up to 60% of the answer
✓ No payment required
✓ Unlock up to 60% of the answer
✓ No payment required
Quick Google sign-in. No separate registration form.