Bioleaching is a microbial process used to extract metals from their ores through the action of microorganisms. It is a key aspect of environmental biotechnology because it provides an eco-friendly alternative to conventional metallurgical processes like smelting, which are energy-intensive and environmentally polluting. Bioleaching exploits the natural ability of certain microorganisms, particularly bacteria and archaea, to oxidize metal-containing minerals, thereby solubilizing the metals for recovery. This process has gained significant attention for the extraction of metals such as copper, gold, uranium, and nickel from low-grade ores and industrial waste. The bioleaching process primarily involves two types of microorganisms: chemolithotrophic bacteria and heterotrophic microorganisms. Chemolithotrophic bacteria, such as *Acidithiobacillus ferrooxidans* and *Leptospirillum ferrooxidans*, derive energy from the oxidation of inorganic compounds like ferrous iron (Fe²⁺) or sulfide minerals. These bacteria play a pivotal role in the leaching of sulfide ores, including chalcopyrite (CuFeS₂), pentlandite ((Ni,Fe)₉S₈), and sphalerite (ZnS). Heterotrophic microorganisms, on the other hand, can produce organic acids, which aid in the solubilization of metals from oxide or silicate ores. For example, *Aspergillus niger* and *Penicillium simplicissimum* produce citric or gluconic acids that facilitate the extraction of metals like zinc and uranium. The bioleaching process can be categorized into two main types: direct and indirect mechanisms. In direct bioleaching, microorganisms attach themselves to the surface of metal sulfide ores and oxidize the mineral directly. This oxidation leads to the formation of soluble metal ions and sulfuric acid, which further promotes metal solubilization. For instance, in copper bioleaching, *Acidithiobacillus ferrooxidans* oxidizes the sulfide component of chalcopyrite to release copper ions into solution. Indirect bioleaching, which is more common, does not require the microorganisms to physically attach to the ore. Instead, they oxidize ferrous ions (Fe²⁺) to ferric ions (Fe³⁺) or sulfide ions to sulfate, which then chemically oxidize the ore. This process is particularly useful for low-grade ores that would otherwise be uneconomical to process using conventional methods. Several factors significantly influence the efficiency and rate of the bioleaching process. One of the most important factors is temperature. Most bioleaching microorganisms are either mesophilic, thriving at temperatures between 25°C and 40°C, or thermophilic, growing optimally between 50°C and