IGNOU MCH 4 2017 - Explain methodology of radioimmunoassay and list its advantages

Question

Explain methodology of radioimmunoassay and list its advantages

28 Mar 2017

Answer :

Word Count : 466

Radioimmunoassay (RIA) is a highly sensitive and specific laboratory technique used to quantify the concentration of antigens, such as hormones, drugs, or other biological substances, in a sample. It combines principles from immunology and radiochemistry to achieve precise measurements. Here's an overview of the methodology of RIA and a list of its advantages:

Methodology of Radioimmunoassay:

1. Antibody Production: The first step in RIA involves the production of highly specific antibodies against the target antigen. These antibodies are generated by immunizing an animal, typically a rabbit or a mouse, with the target antigen. The animal's immune system produces antibodies that recognize and bind to the antigen.

2. Radioactive Labeling: A known quantity of the target antigen is labeled with a radioactive isotope, such as iodine-125 or tritium. This labeled antigen

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