IGNOU CHE 9 2018 - What is genetic engineering? Describe the steps involved in the proces

Question

What is genetic engineering? Describe the steps involved in the process of gene cloning.

25 Jan 2018

Answer :

Word Count : 673

Genetic engineering, also called genetic modification, is the direct manipulation of an organism's genes using biotechnology. It is a set of technologies used to change the genetic makeup of cells, including the transfer of genes within and across species boundaries to produce improved or novel organisms. New DNA is obtained by either isolating and copying the genetic material of interest using recombinant DNA methods or by artificially synthesising the DNA. A construct is usually created and used to insert this DNA into the host organism. The first recombinant DNA molecule was made by Paul Berg in 1972 by combining DNA from the monkey virus SV40 with the lambda virus. As well as inserting genes, the process can be used to remove, or "knock out", genes. The new DNA can be inserted randomly, or targeted to a specific part of the genome.

Gene cloning

Gene cloning involves separation of specific gene or DNA fragments from a donor cell, attaching it to small carrier molecule called vector and then replicating this recombinant vector into a host cell.

Steps involved in gene cloning

Isolation of donor DNA fragment or gene
Selection of suitable vector
Incorporation of donor DNA fragment into the vector
Transformation of recombinant vector into a suitable host cell
Isolation of recombinant host cell

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. Isolation of donor DNA fragment or gene

At first a donor DNA fragment should be isolated. There are two

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