Question
Discuss Edman degradation of N-terminal identification of peptides giving suitable reactions.
Answer :
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Edman degradation is a classical method for identifying the N-terminal amino acid of peptides under mild conditions without disturbing the remaining sequence. In this process, the peptide reacts with phenylisothiocyanate (PITC) in a slightly alkaline medium, similar to how nucleophilic addition reactions occur in Functional Group Organic Chemistry-I. The free amino group at the N-terminus behaves as a nucleophile and attacks the electrophilic carbon of PITC, forming a phenylthiocarbamyl (PTC) derivative. The reaction conditions resemble those used in controlled-phase equilibria, where the pH
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