Question
Discuss briefly any 5 techniques for Isolation & Purification of Constituents.
Answer :
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Isolation and purification of constituents are essential processes in epidemiological research and clinical trials, particularly in the development and standardization of drugs, vaccines, and diagnostic agents. One commonly used technique is solvent extraction, which separates constituents based on their differential solubility in two immiscible solvents. It is frequently applied in isolating bioactive compounds from plant or biological samples prior to pharmacological evaluation in clinical research. Chromatography is another important technique, widely used for separating complex mixtures. Methods such as column
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