IGNOU BBCCT 123 2026 - Explain how DNA foot printing is used to identify promoter r

Question

Explain how DNA foot printing is used to identify promoter regions.

10 Feb 2026

Answer :

Word Count : 235

DNA footprinting is a technique used to identify the specific regions of DNA that are bound by proteins, such as transcription factors or RNA polymerase, helping to pinpoint promoter regions. In this process, a labeled DNA fragment, often radioactively or fluorescently tagged, is incubated with a protein or a mixture of proteins that can bind to specific DNA sequences. The DNA is then partially digested with nucleases, which cleave the DNA at random sites. The key step in DNA footprinting is that when a protein binds to

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