Multiple sequence alignment (MSA) tools, such as Clustal Omega and BLAST, play crucial roles in the field of comparative genomics by facilitating the comparison of genetic sequences across different organisms. These tools are used to uncover evolutionary relationships, identify conserved regions, and predict the function of genes based on sequence similarities. Their role extends across various areas including phylogenetic analysis, structural biology, and functional annotation of genomes. Clustal Omega is an advanced MSA tool known for its ability to handle large datasets with ease. It uses a progressive alignment algorithm, which builds the final alignment by successively aligning smaller groups of sequences, eventually arriving at a global alignment. The tool is highly effective at aligning sequences of varying lengths and divergent evolutionary histories, making it valuable for comparing homologous genes or proteins across species. The output from Clustal Omega not only provides a visual alignment of sequences but also includes a guide tree that helps researchers understand the evolutionary relationships between the sequences. By identifying conserved motifs, domains, or functional sites, Clustal Omega aids in the functional annotation of newly sequenced genomes. BLAST (Basic Local Alignment Search Tool), on the other hand, is a widely used