Question

 

 Describe the unique features of size exclusion chromatography.

01 Mar 2026
Answer :
Word Count : 249
Size exclusion chromatography (SEC), also known as gel filtration chromatography, is a technique used to separate molecules based on their size and shape. The method relies on porous materials, usually silica or agarose, as the stationary phase. These materials contain pores of a specific size that allow smaller molecules to enter and traverse the pores, while larger molecules are excluded and travel faster through the column. The key feature of SEC is that it operates on the principle of molecular sieving. Larger molecules, which are too big to enter the pores, move through the column more quickly
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